DIARECT AG, a privately held company founded in November 1998, specializes in the production and worldwide distribution of key components for development of diagnostic end products.
These crucial components can be proteins, nucleoproteins and nucleic acids that are used as antigens in diagnostic test kits for autoimmune and infectious disease detection. Due to the lack of high-quality antigens from natural sources, we produce these proteins and other components using biotechnological methods. DIARECT’s products are sold in over 25 countries worldwide. DIARECT is also the European distributor for SurModics assay and protein stabilizers, BioFx colorimetric substrates and for chemiluminescence substrates from Lumigen. DIARECT's quality management system is certified according to DIN EN ISO 13485:2003 and ISO 9001:2008
DIARECT’s expertise includes recombinant production of proteins that require expression in a eukaryotic system for proper conformation and activity. The scientists at DIARECT have expert knowledge of the baculovirus/Sf9 expression system for the production of recombinant human auto-antigens; furthermore, E. coli and mammalian cell systems have been established for particular expression tasks (Dr. Thomas Simon and Dr. Heike Schwarz).
The baculovirus/insect cell system is available at fermentation scales of up to 100 L, which allows the production of up to hundreds of milligrams of protein. For expression in E. coli we have a capacity of up to 20-L high density culture for the production of gram amounts of protein. Our upstream processes are complemented by the establishment of cell-free expression using bacterial, insect cell and mammalian systems.